RfaH suppresses sRNA MicA inhibition of fimB
نویسندگان
چکیده
Alexandra Moores, Saranna Chipper-Keating, Lei Sun, Gareth McVicker, Lynn Wales, Krishna 6 Gashi and Ian C. Blomfield* 7 8 School of Biosciences, University of Kent, Canterbury, Kent 9 CT2 7NJ, United Kingdom 10 11 12 * Corresponding author. E-mail: [email protected]; Phone (0)1227 823697; Fax (0)1227 13 763912 14 Running Title: RfaH suppresses MicA inhibition 15
منابع مشابه
RfaH Suppresses Small RNA MicA Inhibition of fimB Expression in Escherichia coli K-12
The phase variation (reversible on-off switching) of the type 1 fimbrial adhesin of Escherichia coli involves a DNA inversion catalyzed by FimB (switching in either direction) or FimE (on-to-off switching). Here, we demonstrate that RfaH activates expression of a FimB-LacZ protein fusion while having a modest inhibitory effect on a comparable fimB-lacZ operon construct and on a FimE-LacZ protei...
متن کاملTranscriptional and Post-Transcriptional Regulation of the Escherichia coli luxS mRNA; Involvement of the sRNA MicA
BACKGROUND The small RNA (sRNA) MicA has been shown to post-transcriptionally regulate translation of the outer membrane protein A (OmpA) in Escherichia coli. It uses an antisense mechanism to down-regulate OmpA protein synthesis and induce mRNA degradation. MicA is genomically localized between the coding regions of the gshA and luxS genes and is divergently transcribed from its neighbours. Tr...
متن کاملExperimental approaches to identify small RNAs and their diverse roles in bacteria – what we have learnt in one decade of MicA research
Nowadays the identification of small RNAs (sRNAs) and characterization of their role within regulatory networks takes a prominent place in deciphering complex bacterial phenotypes. Compared to the study of other components of bacterial cells, this is a relatively new but fast-growing research field. Although reports on new sRNAs appear regularly, some sRNAs are already subject of research for a...
متن کاملRNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA
In Vibrio cholerae, bioinformatic approaches have been used to predict the locations of numerous small RNA (sRNA)-encoding genes, but biological roles have been determined for very few. Here, we describe the expression, processing and biological role of an sRNA (previously known as A10) that was identified through such analyses. We have renamed this sRNA MicX as, like the Escherichia coli sRNAs...
متن کاملCorrection: Small RNA Modules Confer Different Stabilities and Interact Differently with Multiple Targets
Bacterial Hfq-associated small regulatory RNAs (sRNAs) parallel animal microRNAs in their ability to control multiple target mRNAs. The small non-coding MicA RNA represses the expression of several genes, including major outer membrane proteins such as ompA, tsx and ecnB. In this study, we have characterised the RNA determinants involved in the stability of MicA and analysed how they influence ...
متن کامل